discrimination and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer sff-sfr

نویسندگان

مسعود شاه مرادی

کارشناس ارشد علوم باغبانی، دانشکدة کشاورزی، دانشگاه گیلان موسی رسولی

استادیار گروه مهندسی فضای سبز، دانشکدة کشاورزی، دانشگاه ملایر یوسف حمیداوغلی

اعضای هیئت علمی گروه باغبانی، دانشکدة کشاورزی، دانشگاه گیلان علی ایمانی

دانشیار بخش باغبانی، مؤسسة تحقیقات اصلاح و تهیة نهال و بذر کرج رضا فتوحی قزوینی

چکیده

self-incompatibility in almond and prunus species is an important genetic trait that prevents self-fertilization. self-incompatibility in almond is controlled gametophytically by the multiallelic s-locus. this study was done in order to identification and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer sff-sfr.some important morphological traits of parental crosses were evaluated using almond descriptor. also, offspring’s (f1) of five crosses including; a (tuono × 101 genotype), b (supernova × 101 genotype), c( genco × shahrood 21), d (tuono × shahrood 12) and e (tuono × shahrood 17) were tested using pcr in order to dna amplification and self-compatibility evaluation. results of pcr method found that self-compatible progenies showed 449 bp band, while this band not observed in self-incompatible progenies. in addition, all self-incompatible progenies appeared no s1 allele at any condition.

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منابع مشابه

Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer

Self-incompatibility in almond and Prunus species is an important trait that prevents self-fertilization. Selfincompatibility in almond is controlled gametophytically by the multiallelic S-locus. Present study was done in order to identification and preliminary selection of self compatible progenies resulted from controlled crosses in almond using specific primer SfF/SfR. Some important morphol...

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